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Click Chemistry Tools c10269 click it cell reaction buffer kit
C10269 Click It Cell Reaction Buffer Kit, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/click+reaction+buffer/buffer+chemistry+kit+reaction/pm41219002-167-30-36
Average 86 stars, based on 1 article reviews
c10269 click it cell reaction buffer kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Incubation:

Article Title: Differential conformational expansion of NUP98-HOXA9 oncoprotein from nanosized assemblies to macrophases
Article Snippet: Specifically, cells were then permeabilised by incubation with 40 μg/mL digitonin (AppliChem, A1905) for 10 min at room temperature. .. Following two washes with TB, cells were incubated in a dye solution containing 33.3 nM Alexa Fluor 594-H-tetrazine (Click Chemistry Tools) and 66.6 nM LD655-H-tetrazine (Lumidyne Technologies) in TB for 5 min at room temperature. ..

Article Title: Group 1 mGluR stimulation rescues APOE4-mediated translation defects in neurons.
Article Snippet: .. After blocking, neurons were incubated for 3 h with the FUNCAT reaction, following the kit’s instructions to tag AHA-incorporated proteins with an alkynefluorophore Alexa Fluor 555 through the click reaction (C10269; CLICK-iT Cell Reaction Buffer Kit, Click Chemistry Tools). ..

Article Title: Group 1 mGluR stimulation rescues APOE4-mediated translation defects in neurons
Article Snippet: .. After blocking, neurons were incubated for 3 h with the FUNCAT reaction, following the kit’s instructions to tag AHA-incorporated proteins with an alkyne-fluorophore Alexa Fluor 555 through the click reaction ( C10269 ; CLICK-iT Cell Reaction Buffer Kit, Click Chemistry Tools). ..

Article Title: Differential conformational expansion of NUP98-HOXA9 oncoprotein from nanosized assemblies to macrophases.
Article Snippet: Specifically, cells were then permeabilised by incubation with 40 μg/mL digitonin (AppliChem, A1905) for 10min at room temperature. .. Following two washes with TB, cells were incubated in a dye solution containing 33.3 nM Alexa Fluor 594-H-tetrazine (Click Chemistry Tools) and 66.6 nM LD655-Htetrazine (Lumidyne Technologies) in TB for 5 min at room temperature. ..

Sterility:

Article Title: Rainfall-induced microbial resuscitation reveals functional decoupling across biocrust succession
Article Snippet: .. Following acclimation, each microcosm was subjected to a simulated 3 mm rainfall event, receiving 1.36 ml of sterile 1 mM L-homopropargylglycine (HPG) solution (Click Chemistry Tools; Scottsdale, AZ, USA), which corresponds to ∼ 77.5 % of the soil water-holding capacity. ..

Blocking Assay:

Article Title: Group 1 mGluR stimulation rescues APOE4-mediated translation defects in neurons.
Article Snippet: .. After blocking, neurons were incubated for 3 h with the FUNCAT reaction, following the kit’s instructions to tag AHA-incorporated proteins with an alkynefluorophore Alexa Fluor 555 through the click reaction (C10269; CLICK-iT Cell Reaction Buffer Kit, Click Chemistry Tools). ..

Article Title: Group 1 mGluR stimulation rescues APOE4-mediated translation defects in neurons
Article Snippet: .. After blocking, neurons were incubated for 3 h with the FUNCAT reaction, following the kit’s instructions to tag AHA-incorporated proteins with an alkyne-fluorophore Alexa Fluor 555 through the click reaction ( C10269 ; CLICK-iT Cell Reaction Buffer Kit, Click Chemistry Tools). ..

Labeling:

Article Title: Nascent extracellular matrix converts biomaterial cues into cell fate decisions
Article Snippet: .. For EdU incorporation assays, 5 μM EdU was added to chondrogenic AHA media, and incorporated EdU was labeled after fixation using the Click-&-Go® Cell Reaction Buffer Kit (Vector Labs, CCT-1263) and AZDye 647 Azide (Click Chemistry Tools, 1482-1) via copper-catalyzed click chemistry. .. Imaging was performed blindly by selecting random regions using a Leica THUNDER microscope (40× objective), acquiring Z-stacks spanning 100 μm starting from 50 μm below the hydrogel surface.

Article Title: Lysosomal Aspartylglucosaminidase Couples Glycoprotein Catabolism to Cell-Surface GlycoRNA Production
Article Snippet: .. The extracted RNA labeled by MCRs (50 μg, 36 μL) was mixed with 40 μL ‘‘dye-free’’ Gel Loading Buffer II (df-GLBII, thermoFisher) and 4 μL 10 mM stock of DBCO-PEG4-biotin (Click Chemistry Tools, USA). .. Samples were conjugated at 55°C for 10 min in a Thermomixer (Eppendorf) and then purified with as described above.

other:

Article Title: Hydrogels with biodegradable crosslinking
Article Snippet: To a solution of mw=1.6×106 7% DS propylamino HA (0.5 mL of 0.64 mM NH2, 320 nmol NH2) in water was added 0.1 mL of 100 mM PBS, followed by a solution of DBCO-PEG4-NHS ester (Click Chemistry Tools; 0.0308 mL of 25 mM as determined by ε309=13,449 M−1cm−1, 770 nmol, 2.4 equiv) in methanol.



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